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Slide LudwigLab Biocatalysis & Biosensing Research Group

Analytical Laboratory

Specialized equipment for carbohydrate and protein analysis is placed in this area. We identify and quantitate carbohydrates by different high pressure liquid chromatography (HPLC) and capillary electrophoresis (CE) methods. Amino acid composition, trace elements and organic acids in fermentation media, reaction solutions, etc. are also investigated by ion chromatography techniques. The molecular mass and isoelectic point (pI) of proteins are identified by various electrophoretic methods such as SDS-PAGE, native PAGE, isoelectric focusing, chromatofocussing in combination with various staining-techniques or spectrophotometric detectors. The thermodynamic characterization of proteins is studied by isothermal titration calorimetry.

Equipment
Thermo Scientific UltiMate 3000 HPLC
Thermo Scientific Dionex ICS-5000+ Capillary HPIC
Bio-Rad Mini-PROTEAN Tetra Vertical Electrophoresis Cell
Bio-Rad Trans-Blot Turbo Western Blot Transfer System
MicroCal iTC200
SGWater Ultra Clear Basic UV Ultrapure Water System