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Pietro Cannazza

Current position:      MSc Candidate

Project Funding:        LudwigLab

Home University:      UNIMI MILANO, FOOD SCIENCE AND TECHNOLOGY DEPARTMENT

Duration of Stay:       5 months

Research Project:

Structural and functional characterization of cellulose-oxidizing lytic polysaccharide monooxygenases. The research is focused on the interaction between lpmo from Neurospora crassa and cellulosic substrates. the importance of cellulose binding modules (CBMs) will be studied by analyzing  LPMO activity. Through genetic modification of lpmos, different mutants will be produced. The natural protein loops (as CBM) and catalytic site will be modified and removed in order to understand their role in catalytic activity of the enzyme.

During the study enzyme production and purification steps will be optimized. Different culture media will be tested to find the best expression conditions. The manipulated genes will be expressed in Pichia pastoris under the control of methanol inducible AOX1 promoter.

Various chromatography methods are going to be tested and optimized for the purifications steps, depending on the different enzyme that will be produced.

The activity will then be tested on different substrates in various forms like crystalline and amorphous.

Education:

  • Bachelor degree: Food science and technology. Università degli studi di Milano. 2014-2016

Thesis title: Controlling light oxidation flavor in milk by usage oxygen scavenger incorporated in PET resins.

  • Master degree: Food science and technology. Università degli studi di Milano. 2016-2018
  • Research internship in BOKU university. February-July 2018