Daniel Kracher, Ph.D.
Current position: Postdoctoral Researcher
Project Funding: Austrian Science Fund (FWF); European Union
Home University: BOKU University
Duration of Stay: March 2018
Until recently, the degradation of cellulose by fungi has been attributed to a set of diversified cellulases, which hydrolyse the glucan chains in cellulose into soluble sugars. In 2010, the discovery of a fungal oxidative enzyme system that boosts the activity of canonical cellulases changed this enzymatic paradigm. In an extracellular electron transfer process, the flavocytochrome cellobiose dehydrogenase (CDH) activates lytic polysaccharide monooxygenase (LPMO), which initiates the oxidation and cleavage of recalcitrant crystalline cellulose surfaces. The importance of the CDH/LPMO bi-enzymatic system has been shown in several studies and is also obvious from the abundance of lpmo and cdh genes in fungal genomes. The mobility of the CDH cytochrome domain has been frequently linked to LPMO activation, but the molecular basis and kinetics of this interaction await experimental clarification. The main objective of my work is to investigate and visualize domain mobility in the CDH/LPMO system and to correlate the protein dynamics to individual electron transfer steps.
Austrian Science Fund project “Electron Transfer in Cellulose Degrading Enzymes (project I2385)
Erwin-Schroedinger research fellowship of the Austrian Science Fund (“Elektronenfluß beim oxidativen Zelluloseabbau”; project J4154; project leader).
08/2016 - present Postdoctoral researcher in the Ludwig Laboratory
Division of Food Biotechnology, BOKU University, Vienna
07/2016 PhD in Biotechnology (with distinction), BOKU University Vienna Title: “Cellulose degrading oxidoreductases”
12/2009 MSc in Biotechnology (with distinction), BOKU University Vienna
04/2008 BSc in Food- and Biotechnology, BOKU University Vienna
2018 Erwin Schrödinger Fellowship of the Austrian Science Fund
2017 Vincent Massey Award, 19th International Symposium on Flavins and Flavoproteins, Groningen, NED
2017 Klaus Fischer Innovation Award
2017 Best Paper Award (with S. Scheiblbrandner; BOKU University)
2016 Dr. Wilfrieda Lindner Science Award (BOKU University)
2016 Research Award of the Austrian Association of Molecular Life Sciences and Biotechnology (ÖGMBT)
2016 Best poster award – LPMO Meeting Copenhagen, Denmark
2015 Best poster award – 18th month INDOX Meeting, Vienna; 1st place
2015 Best cover award 2014 - 1st place; The Biotechnology Journal
2013 BOKU & tecnet equity Innovation Award - 3rd place
2009 Postgraduate scholarship (BOKU University)