How fungi degrade crystalline cellulose
Daniel Kracher, Alfons Felice, Stefan Scheiblbrandner, Erik Breslmayr
Ninety percent of wood-rotting fungi contain lytic polysaccharide monooxygenases (LPMOs). These powerful enzymes break otherwise inaccessible cellulose surfaces in an oxygen-dependent reaction and initiate their degradation by other enzymes. Such oxidative cellulose degradation greatly contributes to the overall wood-degrading capabilities of fungi and holds great potential to improve the efficiency of commercial enzyme mixtures used for biofuel production. We use biochemical and genomic data to shed light on the activation of LPMOs. This involves either the partner enzyme cellobiose dehydrogenase (1), which directly reduces LPMO, or small redox-active metabolites (2), which can be efficiently recycled by other fungal oxidoreductases (3). Such versatility helps degrader fungi adapt to a range of environmental conditions and is fundamental to fungal physiology.
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- Tan, T.-C. and Kracher, D. et al., 2015. Nature Communications, 6: 7542.
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- Sygmund, C. and Kracher, D. et al., 2012. Applied and Environmental Microbiology, 78: 6161–6171.
Figure from Kracher et al., Science (2016); 352: 1098-1101
|2013||BIOENERGY, European Commission, FP7 People||4 years|
|2013||INDOX, European Commission, FP7||3 years|
|2013||Preseed financing from AWS for DirectSens GmbH||2 years|
|2012||Preseed financing from Accent for DirectSens GmbH||1 years|
|2012||Engineering of bioelement interfaces, BOKU-AIT-NTU Int. Graduate School||3 years|
|2011||3rd generation glucose biosensor, funding agency: Tecnet||1 years|
|2010||Applied research project, Austrian Centre of Industrial Biotechnology (ACIB)||5 years|
|2009||Lactose biosensor project, Research Centre Applied Biocatalysis||6 months|
|2009||Direct electron transfer of redox enzymes, Austrian Academy of Sciences||2 years|
|2009||3D-nanobiodevices, European Commission, FP7||3 years|
|2007||Glucose biosensor, Austrian Science Fund||3 years|
|2006||Novel laccases for biosensors, Research Centre Applied Biocatalysis||3 years|